Single cell DNA methylation analysis has emerged as a groundbreaking technique in the field of epigenetics, offering insights into the DNA methylation patterns of individual cells. Unlike bulk DNA methylation studies, which provide an average profile across a population of cells, single cell DNA methylation allows for the exploration of cellular heterogeneity, revealing how methylation changes can vary between cells in a tissue or organism. This approach has proven invaluable in understanding complex biological processes, such as development, disease progression, and cancer, where different cell types or stages may exhibit distinct methylation signatures. As technologies continue to advance, single cell DNA methylation holds great promise for enhancing our understanding of gene regulation at the single-cell level.